Four Tips for Handcasting SDS-PAGE Gels

Watch this episode of LabNotes for helpful tips like how to pour the stacking and resolving gel at the same time.

For more tips and tricks on protein electrophoresis and protein sample prepartion, and more, download our Guide to Polyacrylamide Gel Electrophoresis and Detection:

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[MuhammadAli-hmhf]

Hi, do you idea for hand-casting of gradient gel by the use of pippet? If yes, do we need to add sucrose in high % gel for the purpose?

[dhrithimallur]

How much 25% Glycerol must be added to the separating gel recipe for a clean separation?

[ricardochagas]

I will try the "double" application...