Ligation with T4 DNA Ligase

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An overview of DNA ligation, followed by a demonstration of the ligation of 2 DNA fragments with T4 DNA Ligase for cloning purposes.
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If you would use a PCR thermocycler, why not set the temp to 4 degrees and then up to 16, and then cycle down again for a few cycles? Wouldnt that give the best results..?

StandardName
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Thanks for the great video. Would you please share the reference for the formula?

tiamheydari
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My DNA is in TE buffer..Must I use water in my ligation reaction?

reubennjue