Leukodepletion Filters Cells As Cell Source-Megakaryocyte Differentiation l Protocol Preview

Leukodepletion Filters-Derived CD34+ Cells As a Cell Source to Study Megakaryocyte Differentiation and Platelet Formation - a 2 minute Preview of the Experimental Protocol

Anaïs Pongerard, Lea Mallo, Christian Gachet, Henri de La Salle, François Lanza, Catherine Strassel
Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS;

This protocol describes in detail all the steps involved in obtaining leukofilter-derived CD34+ hematopoietic progenitors and their in vitro differentiation and maturation into proplatelet-bearing megakaryocytes that are able to release platelets in the culture medium. This procedure is useful for in-depth analysis of cellular and molecular mechanisms controlling megakaryopoiesis.

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