Gal 4 Experiments

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  1. David Patrick Ebertz
  2. Molecular Biology
  3. Vertebrae Development
  4. Technique
  5. Cellular Biology
  6. Cellular Genetics
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Gal 4 Experiments 0:16:55

Gal 4 Experiments

Gal4/UAS System 0:01:35

Gal4/UAS System

GAL4-UAS and LexA-LexAop 0:08:18

GAL4-UAS and LexA-LexAop Explained

Touching mercury 0:00:39

Touching mercury

GAL4-UAS System Application 0:02:01

GAL4-UAS System Application in Functional Genetics | Protocol Preview

elav-Gal4 optogenetics experiment 0:01:06

elav-Gal4 optogenetics experiment control

R64F03-GAL4 x 4Trp's 0:15:01

R64F03-GAL4 x 4Trp's

I tried drinking 0:00:15

I tried drinking 1 Gallon of water every day and this is what happened… ?? #thegallonchallenge

Jumping-GAL4 0:00:19

Jumping-GAL4

bd-Gal4 optogenetics experiment 0:01:06

bd-Gal4 optogenetics experiment control

GAL4-UASgfp in Drosophila 0:03:13

GAL4-UASgfp in Drosophila UMass(MBOMS)

Vertical hydroponic grow 0:00:42

Vertical hydroponic grow tower using 2 inch PVC wye and 5-Gal bucket

Leak testing the 0:00:14

Leak testing the 1 Gal project nano aquarium 🦐 #aquarium #fish #fishtank #shrimp #bettafish #hobby...

How to tear 0:00:10

How to tear a 27-gallon tote bag off of a roll

Kratky Method Experiment 0:05:50

Kratky Method Experiment in 300 gallon IBC container Day 4

drilling foam sample+220 0:00:30

drilling foam sample+220 kg55 gal drum package

20 squash seeds 0:03:00

20 squash seeds planted in 10 gallon planter experiment

nSyb-GAL4 x 4Trp's+trT 0:26:58

nSyb-GAL4 x 4Trp's+trT 4-6 day old females

Potting up Yellow 0:00:21

Potting up Yellow T Rex Seedlings in AutoPots (3.9 gal pot) with an AirBase Square

An Out-Of-This-World Experiment 0:01:32

An Out-Of-This-World Experiment With Space Gal Emily Calandrelli!

Pre spring rock 0:00:15

Pre spring rock hunt @ the Fabulous KeokukGeodes site. 15, 5 gallon buckets of these to dump out 4 U

Gallon pot blow 0:00:30

Gallon pot blow molding machine

Demonstration of Blue 0:04:01

Demonstration of Blue White Screening of Bacterial Colonies - X Gal / IPTG Plates

is this boiling 0:00:44

is this boiling flask really 5 gallon??

Комментарии
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Just thought I'd list my five favorite parts of this video: 

1. "Hold on, let me blow this fly up real quick."
2. "This fly went to McDonald's every day of its life."
3 ."Subsequentially. Subsequentially. Subsequentially."
4. "To look at that a little better without the bloated fly."
5. "Fluorescent things fluoresce. They are shiny and bright and happy."

I love this guy! Please be my teacher! You're funny and smart. Thank you!!!!

UrefutGeonsur
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Thank you very much! This has actually been very helpful to me. :)

lizzy
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Thank you, this helped clarify a lot as I'm too lazy to read the text book.  

pseudonym
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Woo San Diego! Haha great video, savin' my life in biochem

morganwinchell
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thank you very much. IT helped me a lot.

kamalpandey
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David Ebertz Hey, great video! but I'm just wondering, for when you want to express a gene locally in a certain area in an organism that doesnt have Gal4, like in Drosophila (in your example you used the eye), instead of producing 2 constructs, one to produce Gal4, and the second to use Gal4 to produce the protein...why can't you just make one construct, using the transcription factor found in the eye to transcribe the gene of interest? I don't see the requirement for Gal4 in that process? Cheers!

apatta
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Gal4 binds to UAS, a promotor region that is in another strain of flies, that is mated with the Gal4 driver strain.  I'm not sure what you mean with your second construct?... Are you trying to compare tissue specific Gal4, or when Gal4 is expressed in all cells/tissue?

melaniejohnson