How to optimize the fragment hit rate take protein crystals first

'The application of X-ray crystallography as a primary fragment screening method has been underutilized due to the limited availability of a robust system for soaking experiments and solubility issues of fragments.

The major bottlenecks in the experimental setup for crystal soaking are the sensitivity of protein crystals. CrystalsFirst overcomes this limitation using the SmartSoak® technology, which stabilizes protein crystals and enables direct crystallographic screening without trial-and-error optimization of the soaking system.

With a hit rate up to 30% and numerous case studies, we demonstrate the advantages of crystallographic fragment screening as a primary method to access the best possible chemical space.'

[sumalatharani]

Dear Dr. Serghei Glinca,
Very good evening.
My query is, the SmartSoak crystallography technology is the method where you use screens or is there any protocol for Soaking.
I am facing an issue in soaking for one of the target co-crystals with a partial electron density. i have tried soaking the co-crystals up to 100 mM, but i have lost the quality of the crystal which affected the diffraction.
Please do the needful.
Regards,
Sumalatha
Aurigene Oncology PVT, Bangalore, Karnataka, India.