ncbi-blast+ tutorial, blast from the command line (part 1)

This video demonstrates how to use ncbi-blast+. the following commands

are used in the video and the next video:
makeblastdb: used to create a blastdb from a fasta file
blastn: used to do BLAST alignment on a query sequence of nucleic acid against a database of nucleic acid
grep [pattern to search for] [file]
tail -N file (where N is the number of lines from the bottom you want to return)
less [file] display contents of file to terminal

[cen]

This was so incredibly helpful thank you

[RakaRashid]

Hi Brian, can you please give me an idea about doing Blastn search of multiple query files sequentially which are located in the same query folder? How can I do this in a loop in linux command line?

[eshakazi]

Hello, Brian! Your contents are awesome! Loved it!
Could you please tell me if I have a query sequence originating from contig files, but would like to use NCBI ref sequence as the subject sequence, what would the command be for the subject sequence?

[tayfuntasdemir]

Hı, can ı ask a question? I need to extract what I want from the sequence data using the SQL like command. How do I do this?

[paulsalib]

Potentially very dumb question here: is there a reliable way to convert a large number of files from ab1 to fasta so that I can use this pipeline?