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What is Microtutor LIVE?

Introduction to Colocalization in Fluorescence Microscopy

Microtutor: A new virtual light microscopy course

Fluorophore Brightness

Fluorophore Spectra

Photobleaching

Fluorophore Types & Labeling Methods

Optimizing Signal to Noise Ratio

Detectors & Digital Imaging

Fundamental Concepts in Quantitative Microscopy

Trackmate Introduction and Demo

Segmentation & Colocalization

Confocal Microscopy

Imaging Live Specimens

Spherical Aberration

The Confocal Principle

Fluorescence Filters: Imaging Multiple Fluorophores & Dealing with Bleed-through

Fluorescence Filters: Choosing a Filter Set

Signal to Noise Ratio

Microscope alignment for optimal image quality: Koehler Illumination

Best Practices for Pseudo-coloring Fluorescence Microscopy Images

The Point Spread Function

Numerical Aperture

Preventing Objective Lens Damage: Immersion Oil Problems