Production of Monoclonal Antibodies - Hybridoma Technology

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This lecture explains the Production of Monoclonal Antibodies - Hybridoma Technology in simple manner. It includes the following topics
What is Hybridoma technique?
Kohler Experiment
What is Monoclonal Antibody (MAb)?
Principle of Hybridoma Technology
Production of Monoclonal Antibody
Purity and form of immunogen.
Selection of myeloma cell line
Choice of animals
Immunization procedures
Sacrifice of animal and separation of lymphocytes
Immortalization by fusion with tumor cells
Selection of hybridoma cells
Isolation of a monoclonal antibody producing hybridoma cell
Commercial Production of monoclonal Antibodies (MAbs)
  1. Dr. HARIHARAN A.G.
  2. Production of Monoclonal Antibodies
  3. Monoclonal Antibodies
  4. Hybridoma Technology
  5. Kohler
  6. myeloma
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Thank you so much for explaining this topic, it helps me so much to understand the basic and advanced technology of commercial production of monoclonal antibodies

amitrockz