Rare mutation detection by dPCR – Absolute, not relative

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What does a needle in a haystack problem have to do with digital PCR? And how does the nanoplate digital PCR technology makes it easy to find the rare positive as a routine? Watch this video to find out more.

Detecting rare events in complex mixtures or mutant sequences lost in a dense background of wild-type sequences can be difficult, and finding the same rare positive time after time can be even more so. It is indeed like finding a needle in a haystack. But there are other challenges when it comes to detecting rare mutation events in samples. Samples can be quite variable in terms of quality and quantity. There are also different sources of samples – tissue biopsies, urine and stool samples, or blood samples are just a few examples. This situation is common when dealing with liquid biopsy samples and detecting tumor-derived mutant sequences present in tiny amounts in liquid biopsies. There is a solution to this problem: digital PCR! It provides unmatched sensitivity and precision for detecting and quantifying mutations, copy number variations and other genomic events. In this video, our RD expert describes how the QIAGEN nanoplate digital PCR technology of the QIAcuity system applies the power of partitioning to solve such complex problems.
Moreover, the video explains the hyperwell concept unique to digital PCR analysis on the QIAcuity.

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