Introduction to focused ion beam scanning electron microscopy (FIB-SEM)

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This video provides an overview of FIB-SEM, an emerging imaging approach in biology that allows scientists to peer into cells and tissue ultrastructure in 3-D and at resolutions of tens of nanometers. These high-resolution 3-D images are helping researchers reveal structures and processes in cell biology that were previously inaccessible by other imaging methods.

  1. National Cancer Institute
  2. NCI
  3. National Cancer Institute
  4. National Institutes of Health
  5. NIH
  6. microscopy
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By using the FIB to mill away some of the sample to take SEM images to create a 3D image from 2D cross sections, does that destroy the sample? I heard you say that you can redo this to get an information rich image, but I would think the FIB destroys the sample in order to probe the center?

gerardogutierrez