filmov
tv
TechThursday XCV: Illuminator 5000 - aka E. coli Disco💃💫💃
Показать описание
The “Illuminator 5000” is a self-built device by Noah Ritzmann (Müller Lab @ETHZ)👏 that can be used to measure the activity of light-driven proton pumps in bacteria or in artificial membranes. The electronic components are mounted on a custom-built #circuit-board and can be programmed according to the desired needs. An #Arduino inside the device controls which LEDs are turned on and off, while transmitting the data of the pH-meter to a computer, where it can be visualized in real-time.😊
In nature, light-driven proton pumps are produced by tiny marine creatures to harvest energy of the incoming sunlight. They are an interesting topic of research because they can be used to provide energy to artificial systems in form of a proton gradient.
By shining light💡 of different wavelengths onto the sample, the #proton pumps are activated, which changes the pH on both sides of the membrane, they are embedded in. The pH-change outside of the bacteria or artificial compartment can be detected with a pH meter. During the measurement the sample is kept away from ambient light and it is constantly stirred to prevent sedimentation of the cells.
Light of different wavelengths (i. e. different colours) influence the behaviour of the proton-pumps, which can be easily studied in real-time⌚ using the Illuminator 5000. By altering the proton-pumps and/or the properties of the light source, Noah tries to control the proton-pumping directionality and intensity to gain control over downstream processes in more complex molecular systems in the future.
Have a happy Thursday!
#NCCR #NCCRmse #Illuminator #protonpump #pH #light #Ecoli
In nature, light-driven proton pumps are produced by tiny marine creatures to harvest energy of the incoming sunlight. They are an interesting topic of research because they can be used to provide energy to artificial systems in form of a proton gradient.
By shining light💡 of different wavelengths onto the sample, the #proton pumps are activated, which changes the pH on both sides of the membrane, they are embedded in. The pH-change outside of the bacteria or artificial compartment can be detected with a pH meter. During the measurement the sample is kept away from ambient light and it is constantly stirred to prevent sedimentation of the cells.
Light of different wavelengths (i. e. different colours) influence the behaviour of the proton-pumps, which can be easily studied in real-time⌚ using the Illuminator 5000. By altering the proton-pumps and/or the properties of the light source, Noah tries to control the proton-pumping directionality and intensity to gain control over downstream processes in more complex molecular systems in the future.
Have a happy Thursday!
#NCCR #NCCRmse #Illuminator #protonpump #pH #light #Ecoli