Preparing Tris Buffer

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Tris is a buffer that's used to maintain a stable pH when working with solutions in the lab.
In this video, we'll demonstrate the steps for preparing 250 mL of a 0.5 molar Tris buffer.

We'll measure our powder, mix it with water using a stir plate, and then test our pH with a pH meter. We'll add drops of hydrochloric acid to lower the pH to our target of 7.8.

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Did you know that TRIS will form precipitate with Ag+ in conventional pH meter electrode?
One does not simply measure TRIS buffer in pH meter with Ag/AgCl electrode with single junction reference electrode (mostly used in conventional pH meter now days) because it will poisons the reference electrode.
using calomel electrode or double junction reference electrode is the correct way

it seems that your using single junction electrode (i can see it clearly) and therefore your actual pH in not 7.8.

ZulhilmiHusni

Great video, very helpful, but I think your N on the NaOH bottle is backwards.

nathancoleman

no calculations mentioned, how do we know we need to add 15.14g of powder? where does it come from

arshiaamjad

The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer

onnorokom

how can we know to add 15.4g of tris buffer in sample water

dmadhav

Why we only use HCl and not any other acid for stabilizing ph please answer

the-spiritual-thoughts

can you make a video about how to prepare lysis buffer

aminaferielkadri

I had no idea that you could add HCl to your buffer to alter the PH. my mind is blown.

bbmeesci

I have a question, if I want to prepare a solution at a concentration of 100 mM pH: 7.8 from this solution(0.5M, pH:7.8), I have to re-measure your pH? Thanks for you video.

coca

When i add the tris my solution becomes cloudy. I have to adjust the pH with hcl. Only after it will be clear again. I'm using 1 g/l of tris(THAM). Is this normal?

XxXnonameAsDXxX

Thank you for the Video, it's really helpful. What we put instead of HCl to increase our pH Buffer solution?, for example if my buffer pH is 8, 5 but I want it pH at 9.0

erygusman

good video, ,thanks alot, , , but can you help me about the best buffer that can be used in my experiment when my drug is unstable at phosphate buffer, , , , means that when i prepared the phosphate buffer the uv absorbance was not stable that changed with time but i need the same absorbance for 14 days !!!! so what the solution ?? plz help me :((

monsabas