COVID-19 Insights: Tests for SARS-COV-2 (COVID-19)

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. Our notes

SARS-COV-2 testing

An example test publication

E Gene (envelop)
N Gene (nucleocapsid)
ORF1b of RdRp (open reading frame 1b of the RNA dependent RNA polymerase.)

An example testing company

Taqman process

Polymerase chain reaction

Thermal cycler machine

Overview of ELISA

Disclaimer:
This video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. The content provided in this video is for informational and educational purposes only.
Please consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. No information in this video should ever be considered as a substitute for advice from a healthcare professional. ...
Disclaimer:
This video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. The content provided in this video is for informational and educational purposes only.
Please consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. No information in this video should ever be considered as a substitute for advice from a healthcare professional.

[ritupandey]

Thank you so much. You described so well.

[evanszoogah]

Very simple and well understood lecture.

[sanketpanchal]

Vaccination lecture please If we make antibody entire corona virus problem will be solve... We need to focus on it.... Thank you sir..
Sir you are truly make a doctor's.. Sir You are great teacher And great doctor as

[tmk]

Again thank you Dr. Mobeen. And if you ate discussing HCQ tomorrow, please share you views regarding another mode of viral pathogenesis, and that is it binds with heme molecule and releases ion fe, which is highly toxic especially to the lung. Thats why there is increase Hb% and ferritin in Covid-19 patients etc and HCQ or Chloroquine interact with its binding to

[fredmad]

Dr Been, which is more accurate blood test or pcr test through the nasal?

[mohamed]

Please, could you explain how to read results and graphic images 🙏🙏🙏

[jrhjrh]

Maybe some can answer this these question 🤔.
What does mean??
Sars-cov-2 Detected followed by reference range: not detected. The hospital said my freind also has bacterial pneumonia. Would a bacterial swab been taken to tell you what kind of bacteria?? Im a laymen I know most of you are well versed. Many thanks in advance.

[MdMazidulHaque]

Thanks a lot for your valuable time and inspiration.

[sharadbholane]

Dr sir Salute nThaks a lot it's our pleasure to learning more

[gforce]

Amazing video, thank you for your time Dr. Mobeen

[debasmitadas]

rna specific to sars-cov-2 detected positive, what does it mean????

[mohamedabdirahman]

mohamed from kenya -very nice lecture mashaallah

[saganworshipper]

I am currently in a war with a bunch of the "open it all up NOW" crowd. How can I explain to them in the most simple terms possible as to why we need testing on a grand scale if we are to reopen the economy as safely as possible? I don't know how to put it into words so they can understand.

[fabriennern]

Such a blessing! Would really be great if they con do mass testing as soon as possible.
Does anyone know where can I buy Covid-19 Antibody testing kit?

[alangill]

An excellent video like always! Quick question, there is also a rapid immunoassay test, which you can do yourself at home using your blood and a buffer and you get your results within 10 minutes. Here in the UK, there has been a lot of talk about it as a lot of people are receiving false negatives due to testing it 1-2 days post symptoms. My question is what is your opinion on these rapid tests in terms of accuracy, and when should you ideally use this test, I would assume 5 days post symptoms?

[tnutss]

If it takes up to 7 days... I heard even up to 12 days for results from California governor.. wouldn’t the virus be deteriorated by then? Would it be valid after a certain time frame? Talking about pcr test here

[nagamallibanavath]

All over countries, repeat infection in the case person, it means might be this virus has combination of double virus, one fading and 2nd one showing up I don't know, might be posdible.
Just guess

[masoodahmed]

Sir, How can we differentiates the Viral RNA and Human RNA during PCR?
we are taking swab sample it may also contain our cells and RNA., , , ,

[markovichglass]

How does one isolate the RNA from other genetic material? How do we know the RNA is the exact virus RNA and not some other genetic material found in the test sample? When the rt-pcr transcripts and makes c-DNA, is this -c-DNA tested for its genetic material or if its DNA, it glows and the machine counts how many compounds that are fluorescence? Thank you kindly sir
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The virus sample is removed from the nasal area, then placed in a tube and this tube at the lab gets primers and enzymes added, the reverse transcriptase makes a copy of said RNA to make C-DNA. Is the primer and enzyme virus specific or can it transcribe any genetic material, and what if there is more then one type of genetic material in the sample, can your deviate between different genes?

[kramalin]

This covid19 has it pass the Koch postulates? Tq Dr