Ask a Scientist: What is Duplication Rate in NGS?

How does duplication rate affect the quality of NGS data, and what can be done to minimize duplicate reads? This video describes what duplicate reads are and how they reduce the quality of sequencing data, and also identifies potential sources of duplicate reads throughout the next-generation sequencing workflow. In addition, it provides tips that can be implemented at several different steps in the NGS workflow— including hybridization-based target enrichment— for reducing duplicate reads and improving data quality.

Presented by Dustin Masser, Roche Sequencing and Life Science

MC-US-08231