Fluorescent in situ hybridization (FISH)

This gene technology lecture explains about fluorescent in situ hybridization or FISH and the role of FISH in genome mapping.
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Download the study materials here-

[aali]

I dont know if im correct, but is this what happens in FISH:
1. flourescent dye on a probe DNA
2. denaturation of the probe and target DNA (single strands)
3. Then getting the probe single stranded dna to become complementary to the target dna (hybridization, i think?)
4. detecting the flourescent dye on the dna

[monicathukkaramnt]

pop up messages in your videos are quite annoying, please add your additional services in the description.

[rakshiichaudhary]

Didn't get the concept of making a probe? How it becomes specific to our required sequence? How exactly we do it?

[nehanasir]

How is the complementary strand to the target sequence removed before hybridization
Because it may happen that during hybridization or cooling, the target strand again bind to the complementary sequence instead of probe

[prikwilson]

Nice demo, except those pop ups..also, please mention washing away the excess probes and excess flourescent label molecules.

[namratashah]

can you please make a video explaining the chromosome painting

[yeswebuy]

thanks it helped a lot to understand FISH (y)

[PaolaAguirreru]

...i never said it before, but I love your Indian accent <3 .. haha thanks a lot!

[Duuueewd]

What is the point of having to fluorescent molecules? in your animation you mention that both the purple and the yellow circles are fluorescent

[aksshorthand]

Actually where is the gene of interest taken from...?? Can u just give me ur piece of mind

[neetikothari]

In the beginning the ones tht get attached to dna are probes or marker ??

[bijaymuduli]

Hello sir
How to write reproduction of paramecium for sexual reproduction

[beltrantorres]

due you seriously need to cut back on those pup up links non stop
its very very annoying

[MrTote]

Disliked because of the pop up messages.