3D Spheroid Cultures of Cancer Cells Cell Viability Assessment | Protocol Preview

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells - a 2 minute Preview of the Experimental Protocol

Michala G. Rolver, Line O. Elingaard-Larsen, Stine F. Pedersen
University of Copenhagen, Section for Cell Biology and Physiology, Department of Biology, Faculty of Science;

Here, we present several simple methods for evaluating viability and death in 3D cancer cell spheroids, which mimic the physico-chemical gradients of in vivo tumors much better than the 2D culture. The spheroid model, therefore, allows evaluation of the cancer drug efficacy with improved translation to in vivo conditions.

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