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Acid Fast Staining | ZN Staining and Kinyoun Staining for Mycobacterium tuberculosis
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In this video , we are discussing Principle, Procedure and Result interpretation of ZN staining (Ziehl Neelsen staining) adn Kinyoun staining method used for Mycobacterium species i.e., Mycobacterium tuberculosis and Mycobacterium leprae.
Acid fastness is resistance to decolorization by acids which is major property shown by Mycobacterium specie and other cells.
Mycobacterium tuberculosis have Mycolic acid in their cell wall, that don't allow normal stain used in microbiology to stain the cell. in order to stain these cells, acid fast staining techniques are developed that includes ZN staining and Kinyoun staining.
Timecodes
0:00-intro
0:28-Acid fastness and acid fast techniques
1:36-Types of Acid fast staining techniques
2:20-why to perform acid fast technique for Mycobacterium
3:17-Principle of ZN staining
7:06-strongly acid fast
7:48-weekly acid fast
8:13-modified ZN staining (Kinyoun method)
9:06-procedure of ZN staining and Kinyoun staining
11:32-result intrepretation
12:54-smear examination and reporting AFB seen
15:00-No AFB seen
Acid fastness is resistance to decolorization by acids which is major property shown by Mycobacterium specie and other cells.
Mycobacterium tuberculosis have Mycolic acid in their cell wall, that don't allow normal stain used in microbiology to stain the cell. in order to stain these cells, acid fast staining techniques are developed that includes ZN staining and Kinyoun staining.
Timecodes
0:00-intro
0:28-Acid fastness and acid fast techniques
1:36-Types of Acid fast staining techniques
2:20-why to perform acid fast technique for Mycobacterium
3:17-Principle of ZN staining
7:06-strongly acid fast
7:48-weekly acid fast
8:13-modified ZN staining (Kinyoun method)
9:06-procedure of ZN staining and Kinyoun staining
11:32-result intrepretation
12:54-smear examination and reporting AFB seen
15:00-No AFB seen
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